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Startat av Mayordomo, 2009-10-09, 11:53
CitatUpon re-reading three of the four Retrovirology papers it became clear to me that they show that identification of XMRV can be fraught with contamination problems, but they do not imply that previously published studies are compromised by these findings.
CitatA strong argument that the novel human retrovirus XMRV is not a laboratory contaminant is the the finding that viral DNA is integrated in chromosomal DNA of prostate tumors.
CitatAnimals sacrificed at the acute stage showed evidence of viral replication in spleen, lung, lymph nodes and liver. In contrast, sacrifice of 2 animals at 19 weeks post XMRV re-inoculation showed greater dissemination of XMRV DNA and RNA in various organs including the GI and urinary tract as well as in vaginal tissue of the one female. By Western blot analysis, all 3 chronically infected macaques developed antibody responses to env and gag proteins
Citat...the plasma viral load was undetectable
Citat"All this study really says is that we can't detect it in the blood reproducibly," says Judy A. Mikovits, PhD, director of research at the Whittemore Peterson Institute in Reno, Nev."The interpretation says that it's not there or that it's not a human infection, and there's no data in this study or any other to support that," she says.
CitatIn conclusion, our studies demonstrated that several MLVstrains were present in over one fourth of xenograft cell lines.Infected cell lines were identified in most laboratories workingwith or establishing xenograft cultures, indicating that suchcontamination was widespread. Infected cultures usually releaselarge numbers of infectious virions, and intra-laboratory spreadof MLV virus to other cell lines maintained in the same facilitiesmay occur, confirming the highly infectious nature of MLVvirus.